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Overview
We provide customized immunohistochemistry and nucleic acid detection services:
- In depth consultations to assess staining strategies. Includes customized antibody and control tissue searches -- many human control tissues and cell lines are already available
- Chromogenic or fluorescent detection methods offered
- Custom FISH services for any genomic target(s) of interest
- Multiplex staining available
Fees
Investigator supplies primary antibodies unless the IHC core already has the primary optimized. There are no guaranteed results from using this service. Fees will be accessed on work done and not outcome. Please provide primary antibody spec sheets with requisition. For best results please have your tissue processed and sectioned in our TMA/Histology Core facility. Please note that prices are subject to change and you should contact the lab to verify pricing. These prices are for JHU affiliates. All others should inquire with Helen Fedor (hfedor@jhmi.edu).
| Consultation | |
| Initial consultation (per 1/4 hour)1 | $25.00 |
| Additional consultation (per 1/4 hour)2 | $50.00 |
| IHC/IF | |
| Full antibody work up3 | $500.00 |
| Partial antibody work up4 | $250.00 |
| Co-staining work up |
Inquire |
| Single antibody immunostain (per slide) | $35.00 |
| Dual antibody immunostain (per slide) | $45.00 |
| FISH | |
| FISH work up - commercial probe (probe not incl) | $250.00 |
| FISH work up including custom in-house probe | $500.00 |
| FISH (per slide) | $50.00 |
| Control slide- normal human metaphase | $10.00 |
| Controls | |
| Control slide - single tissue | $8.50 |
| Control slide - multi tissue array | Inquire |
| Custom cell line array | Inquire |
| Misc. | |
| Flat slide holder | $8.50 |
| Microscope consult (per 1/4 hr.) | $25.00 |
| Fluorescence microscope user fee (per 1/4 hr.) | $25.00 |
2) Additional consultation = assistance in finding antibodies, BACs, appropriate controls, etc.
3) For antibody with unknown performance
4) For antibody with some knowledge of performance (e.g. on different cell line/tissue type)
Forms
- IHC Work Request Form
Use this form to make a work request for
our customized immunohistochemistry and nucleic acid detection services.
Contact
Location: Bond St Annex, Room 305 Basement
For inquiries, please contact:
Dr. Alan Meeker, Ph.D.
Email: ameeker@mail.jhmi.edu
Phone: 410-502-3398